Ozonated Water Research and Approvals

Lab Testing of Ozonated Water
Sponsored lab test results from BoiE Always Ready devices confirm ozone's ability to destroy bacteria and viruses in as little as 30 seconds. Ozonated water has been scientifically proven to disinfect even at relatively low concentrations with appropriate contact time.

 Microbes Tested¹

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MS2 phage ATCC 15597-B1 (Virus)

99.9999%

30 sec

BoiE

¹Claim: Antiviral effectiveness against a non-enveloped virus that is harder-to-kill than the SARS-CoV-2 enveloped virus that causes COVID-19. Double replicate testing performed at Microchem Laboratory, Round Rock, TX on 6/30/2021. Standard: ASTM E2315 Liquid Suspension Time Kill Test.
 Microbes Tested²

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Escherichia coli (E.coli)

99.9999%

30 sec

BoiE

Staphylococcus aureus (Staph)

99.9999%

30 sec

BoiE

²Claim: For use as a food-contact sanitizer on hard, non-porous surfaces. Double replicate testing performed at Microchem Laboratory, Round Rock, TX on 4/29/2021. Standard: AOAC 960.09 Germicidal and Detergent Sanitizing Action of Disinfectants.
 Microbes Tested³

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Staphylococcus aureus

>99.99%

30 sec

BoiE

Klebsiella pneumoniae

>99.99%

30 sec

BoiE

Listeria monocytogenes

>99.99%

30 sec

BoiE

Salmonella typhii

>99.99%

30 sec

BoiE

³Claim: For use as a non-food-contact sanitizer on hard, non-porous surfaces. Testing performed at Microchem Laboratory, Round Rock, TX on 2/11/2021. Standard: ASTM E1153 Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces.

Aqueous Ozone Approvals
Aqueous Ozone is approved by the EPA, FDA, USDA, is considered GRAS, and is compliant with the EPA Organic Program as a natural and effective cleaner and sanitizer.

  • The FDA approved Aqueous Ozone as an antimicrobial agent in 2001
  • USDA/National Organic Program (NOP) Ozone Approved
  • Nonfood Compounds Program listed on White List as a no-rinse sanitizer and cleaner
  • GRAS and compliant with the EPA Organic Program


Identifying Aqueous Ozone Concentration
Ozone concentration in water from 0.0 to 1.5 ppm can be confirmed using commercially available ozone test strips. Using an ozone test strip is simple: Gently dip the test strip into the ozonated water for 5 seconds with constant, gentle back and forth motion (10 half strokes). Remove the strip and shake once briskly to remove excess water. Wait 30 seconds then match the strip to the color chart. A minimum concentration of 0.25 ppm is all that is required for disinfection with sufficient contact time. These ozone test strips are availble for purchase when ordering.

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