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Ozonated Water Research and Approvals
Lab Testing of Ozonated Water
Sponsored lab test results from BoiE Always Ready devices confirm ozone's
ability to destroy bacteria and viruses in as little as 30 seconds. Ozonated water has been scientifically proven
to disinfect even at relatively low concentrations with appropriate contact time.
| Microbes Tested¹ |
Reduction |
Contact |
Sponsor |
| MS2 phage ATCC 15597-B1 (Virus) |
99.9999% |
30 sec |
BoiE |
| ¹Claim: Antiviral effectiveness against a non-enveloped virus that is harder-to-kill than the SARS-CoV-2 enveloped virus that causes COVID-19. Double replicate testing performed at Microchem Laboratory, Round Rock, TX on 6/30/2021. Standard: ASTM E2315 Liquid Suspension Time Kill Test. | |||
| Microbes Tested² |
Reduction |
Contact |
Sponsor |
| Escherichia coli (E.coli) |
99.9999% |
30 sec |
BoiE |
| Staphylococcus aureus (Staph) |
99.9999% |
30 sec |
BoiE |
| ²Claim: For use as a food-contact sanitizer on hard, non-porous surfaces. Double replicate testing performed at Microchem Laboratory, Round Rock, TX on 4/29/2021. Standard: AOAC 960.09 Germicidal and Detergent Sanitizing Action of Disinfectants. | |||
| Microbes Tested³ |
Reduction |
Contact |
Sponsor |
| Staphylococcus aureus |
>99.99% |
30 sec |
BoiE |
| Klebsiella pneumoniae |
>99.99% |
30 sec |
BoiE |
| Listeria monocytogenes |
>99.99% |
30 sec |
BoiE |
| Salmonella typhii |
>99.99% |
30 sec |
BoiE |
| ³Claim: For use as a non-food-contact sanitizer on hard, non-porous surfaces. Testing performed at Microchem Laboratory, Round Rock, TX on 2/11/2021. Standard: ASTM E1153 Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces. | |||
Aqueous Ozone Approvals
The use of aqueous ozone is approved by the EPA, FDA, USDA, is considered GRAS, and is compliant with the EPA Organic
Program as a natural and effective cleaner and sanitizer. Please note
that government agencies do not endorse products.
- The FDA approved Aqueous Ozone as an antimicrobial agent in 2001
- USDA/National Organic Program (NOP) Ozone Approved
- Nonfood Compounds Program listed on White List as a no-rinse sanitizer and cleaner
- GRAS and compliant with the EPA Organic Program
Identifying Aqueous Ozone Concentration
Ozone concentration in water from 0.0 to 1.5 ppm can be confirmed using
commercially available ozone test strips. Using an ozone test strip is simple: Gently dip the test strip into the
ozonated water for 5 seconds with constant, gentle back and forth motion (10 half strokes). Remove the strip and
shake once briskly to remove excess water. Wait 30 seconds then match the strip to the color chart. A minimum concentration
of 0.25 ppm is all that is required for disinfection with sufficient contact time. These ozone test strips are
available for purchase when ordering.
